HURP localization in metaphase is the result of a multi-step process requiring its phosphorylation at Ser627 residue

Faithful chromosome segregation during cell division requires accurate mitotic spindle formation. As mitosis occurs rapidly within the cycle, proteins involved in assembly undergo rapid changes, including their interactions with other proteins. The proper localization of HURP protein on kinetochore fibers, close proximity to chromosomes, is crucial for ensuring congression and chromosomes. In this study, we employ photoactivation FRAP experiments investigate impact alterations microtubule flux phosphorylation at Ser627 residue its dynamics. Furthermore, through immunoprecipitations assays, demonstrate various proteins, such as TPX2, Aurora A, Eg5, Dynein, Kif5B, Importin β , mammalian cells mitosis. We also find that regulates interaction these partners Our findings suggest participates least two distinct complexes metaphase ensure thereby promoting bundling stabilization fibers.